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Apoptosis analysis of melanoma cells following treatment with selected complexes, compared to untreated controls. ( A ) Quantitative assessment of A-375 and SK-MEL-28 cells treated with Cu(b-TSC) 2 , and G-361 cells treated with Pd(dm-TSC-pCl) 2 at their respective IC 50 concentrations. Cells were stained with Annexin V-CF488A, Hoechst 33342, and propidium iodide (PI) and analysed using the NucleoCounter ® NC-3000 ™ image cytometer. The bar graph represents the percentage of live (Annexin V-negative/PI-negative), early apoptotic (Annexin V-positive/PI-negative), late apoptotic (Annexin V-positive/PI-positive), and necrotic cells. (B) Representative scatter plots for each condition, illustrating the distribution of cell populations divided into four quadrants: lower left (Q1ll), viable cells; lower right (Q1lr), early apoptotic cells; upper right (Q1ur), late apoptotic cells; and upper left (Q1ul), necrotic cells.

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Figure Lengend Snippet: Apoptosis analysis of melanoma cells following treatment with selected complexes, compared to untreated controls. ( A ) Quantitative assessment of A-375 and SK-MEL-28 cells treated with Cu(b-TSC) 2 , and G-361 cells treated with Pd(dm-TSC-pCl) 2 at their respective IC 50 concentrations. Cells were stained with Annexin V-CF488A, Hoechst 33342, and propidium iodide (PI) and analysed using the NucleoCounter ® NC-3000 ™ image cytometer. The bar graph represents the percentage of live (Annexin V-negative/PI-negative), early apoptotic (Annexin V-positive/PI-negative), late apoptotic (Annexin V-positive/PI-positive), and necrotic cells. (B) Representative scatter plots for each condition, illustrating the distribution of cell populations divided into four quadrants: lower left (Q1ll), viable cells; lower right (Q1lr), early apoptotic cells; upper right (Q1ur), late apoptotic cells; and upper left (Q1ul), necrotic cells.

Article Snippet: Cell cycle analysis was performed using a NucleoCounter NC-3000 (ChemoMetec, Allerød, Denmark).

Techniques: Analysis, Staining, Cytometry